FOB Price
Get Latest Price5 ~ 5 / Milliliter
|100 Milliliter Minimum Order
Country:
China
Model No:
-
FOB Price:
5 ~ 5 / Milliliter Get Latest Price
Place of Origin:
-
Price for Minimum Order:
5 per Milliliter
Minimum Order Quantity:
100 Milliliter
Packaging Detail:
-
Delivery Time:
7-15 days if in stock
Supplying Ability:
1000 Milliliter per Month
Payment Type:
T/T
Product Group :
-
Product: 2*Taq PCR Master (without dye)
Cat. No.: T***4
Price: $5/ml (negotiable if bulk purchase)
Storage conditions: **0℃
Product description
The concentration of Taq-ASPCRMix is 2×. It is easy and quick to
use and can reduce contamination during PCR operation. When using,
just take an appropriate amount of 2×Taq-AS PCR Mix, add template
and primers, and add ddH2O to make up the volume, so that the
concentration of the reaction system is 1×, and then you can carry
out PCR reaction. The 3' end of the PCR product has a prominent A
base and can be directly used for T/A cloning after
purification.
The TaqDNA polymerase in this Mix is a screened Taq-AS (Advanced
Strong) DNA Polymerase mutant, which can efficiently amplify DNA
fragments ≤5kb and has good inhibitor tolerance. Its amplification
speed is about 3 times that of ordinary Taq DNA polymerase, so it
can greatly reduce the time required for the PCR extension process,
thereby shortening the entire PCR reaction. Different from the fast
Taq polymerase based on the fusion protein principle, the use of
Taq-AS is closer to WT-Taq, and it is not easy to have
electrophoretic band diffusion, dragging or fragment size
changes.
Quality control
Nuclease activity detection
*5μl 2×Taq-AS PCR Mix and **0ng supercoiled plasmid DNA were
prepared into a *0μl reaction system. After incubation at *7℃ for
4h, less than *0% of the plasmid DNA was converted into a nicked or
linear state by agarose gel electrophoresis.
Nonspecific nuclease activity detection
*5μl 2×Taq-AS PCR Mix and *5ng double-stranded DNA fragment were
prepared into a *0μl reaction system. After incubation at *7℃ for
*6h, agarose gel electrophoresis was used to detect that there was
no change in the double-stranded DNA substrate.
Country: | China |
Model No: | - |
FOB Price: | 5 ~ 5 / Milliliter Get Latest Price |
Place of Origin: | - |
Price for Minimum Order: | 5 per Milliliter |
Minimum Order Quantity: | 100 Milliliter |
Packaging Detail: | - |
Delivery Time: | 7-15 days if in stock |
Supplying Ability: | 1000 Milliliter per Month |
Payment Type: | T/T |
Product Group : | - |