Nitrofuran (AMOZ) ELISA Test Kit

Product Description

Nitrofuran (AMOZ) ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the
sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample
and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ
antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This
value is compared to the standard curve and the AMOZ concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Tissue(Chicken, duck, porcine meat, beef and mutton).......................0.2 ppb
Liver(Chicken, duck, porcine meat, beef and mutton)..........................0.2 ppb
Honey, milk, intestine............................................................................0.2 ppb
Fish, shrimp(some interference)...........................................................0.3 ppb
Recovery rate
Tissue, liver......................................................................................... *0±*5%
Honey, milk, intestine.......................................................................... *0±*5%
Cross-reaction rate
AMOZ...................................................................................................... **0%
AHD....................................................................................................... <0.1%
AOZ........................................................................................................<0.1%
SEM....................................................................................................... <0.1%
3. Components
1） Micro-well strips: *2 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3） Enzyme conjugate (7 mL)................................................................... red cap
4） Antibody working solution (7 mL).......................................................blue cap
5） Substrate A solution (7 mL).............................................................. white cap
6） Substrate B solution (7 mL)..............................................................black cap
7） Stop solution (7 mL)....................................................................... yellow cap
8） *0× concentrated washing buffer (*0 mL)....................................... white cap
9） 2× concentrated redissolving solution (*0 mL)...................... transparent cap
*0） *-Nitrobenzaldehyde (C7H5NO3)(*0 mL)......................................... white cap

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