Product Description
INTENDED
USE
ThisB.GAGEs
ELISA kit is intended Laboratory for Research use only and is not
for use in diagnostic or therapeutic procedures. The Stop
Solution changes the color from blue to yellow and the intensity
of the color is measured at **0nm using a spectrophotometer. In
order to measure the concentration of AGEs in the sample, this
AGEs ELISA Kit includes a set of calibration standards. The
calibration standards are assayed at the same time as the samples
and allow the operator to produce a standard curve of Optical
Density versus AGEs concentration. The concentration of AGEs in
the samples is then determined by comparing the O.D. of the
samples to the standard curve.
PRINCIPLE OF THE
ASSAY
The coated well
immunoenzymatic assay for the quantitative measurement of serum
AGEs utilizes a monoclonal anti-AGEs and a AGEs-HRP conjugate.
The assay sample and buffer are incubated together with anti-AGEs
antibody coated plate for sixty and washed. The diluted AGEs-HRP
conjugate is then added to each well and incubated. After the
incubation period, the wells are decanted and washed three times.
The wells are then incubated with a substrate for the enzyme. The
product of the enzyme-substrate reaction forms a blue colored
complex. Finally, a stopping solution is added to stop the
reaction, which will then turn the solution yellow. The intensity
of color is measured spectrophotometrically at **0nm in a
microplate reader. The intensity of the color is inversely
proportional to the AGEs concentration since AGEs from samples
and AGEs-HRP conjugate compete for the anti-AGEs antibody binding
site. Since the number of sites is limited, as more sites are
occupied by AGEs from the sample, fewer sites are left to bind
AGEs-HRPconjugate. Standards of known AGEs
concentrations are run concurrently with the samples being
assayed and a standard curve is plotted relating the intensity of
the color (Optical Density) to the concentration of AGEs. The
unknown AGEs
concentration in each
sample is interpolated from this curve.
REAGENTS
PROVIDED
All reagents
provided are stored at **8° C. Refer to the expiration date on
the label.
1. MICROTITER
PLATE *6 wells
2. ENZYMECONJUGATE 6.0
mL 1 vial
3. STANDARD.1
0pg/ml 1 vial
4. STANDARD.2
*0pg/ml 1 vial
5. STANDARD.3 **0pg/ml 1
vial
6. STANDARD.4 **0pg/ml 1
vial
7. STANDARD.5 **0pg/ml 1
vial
8. STANDARD.6 ***0pg/ml 1
vial
9. SUBSTRATE
A 6.0 mL 1 vial
*0. SUBSTRATE
B 6.0
mL 1 vial
*1. STOP
SOLUTION 6.0 mL 1 vial
*2. WASH
SOLUTION x**0 *0 mL 1 vial
*3. Instruction
1
SAMPLE COLLECTION
AND STORAGE
Serum-Use a serum separator tube(SST)
and allow samples to clot for *0minutes before centrifugation for
*5minutes at approximately ***0xg. Remove serum and assay
immediately or aliquot and store samples at **0 °C or
**0°C.
Plasma- Collect plasma using EDTA or
heparin as an anticoagulant. Centrifuge samples for *5 minutes at
***0 x g at **8°C within *0minutes of collection. Store samples
at **0°C or **0°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid
and other biological fluids- Remove particulates by
centrifugation and assay immediately or aliquot and store samples
at **0°C or **0°C.Avoid repeated freeze-thaw cycles.
NOTE:Serum, plasma, and cell culture
fluid samples to be used within 7 days may be stored at **8°C,
otherwise samples must stored at **0°C(≤2months) or
**0°C(≤6months) to avoid loss of
bioactivity and contamination. Avoid freeze-thaw cycles .When
performing the assay slowly bring samples to room
temperature.
DO NOT USE
HEAT-TREATED SPECIMENS.
MATERIALS REQUIRED
BUT NOT SUPPLIED
1. Microplate
reader capable of measuring absorbance at **0 nm.
2. Precision
pipettes to deliver 2 ml to 1 ml volumes.
3. Adjustable
*0ml ***0ml pipettes for reagent preparation.
4. Adjustable
*0ml ***0ml pipettes for reagent preparation.
5. **0
ml and 1 liter graduated cylinders.
6. Calibrated
adjustable precision pipettes, preferably with disposable plastic
tips. (A manifold multi-channel pipette is desirable for large
assays.)
7. Absorbent
paper.
8. *7°C
incubator.
9. Distilled
or deionized water.
*0. Data
analysis and graphing software. Graph paper: linear (Cartesian),
log-log or semi-log, or log-logit as desired.
*1. Tubes
to prepare standard or sample dilutions.
PRECAUTIONS
1. Do
not substitute reagents from one kit lot to another. Standard,
conjugate and microtiter plates are matched for optimal
performance. Use only the reagents supplied by
manufacturer.
2. Allow
kit reagents and materials to reach room temperature (****5°C)
before use. Do not use water baths to thaw samples or
reagents.
3. Do
not use kit components beyond their expiration date.
4. Use
only deionized or distilled water to dilute reagents.
5. Do
not remove microtiter plate from the storage bag until needed.
Unused strips should be stored at **8°C in their pouch with the
desiccant provided.
6. Use
fresh disposable pipette tips for each transfer to avoid
contamination.
7. Do
not mix acid and sodium hypochlorite solutions.
8. Serum
and plasma should be handled as potentially hazardous and capable
of transmitting disease. Disposable gloves must be worn during
the assay procedure, since no known test method can offer
complete assurance that products derived from human blood will
not transmit infectious agents. Therefore, all blood derivatives
should be considered potentially infectious and good laboratory
practices should be followed.
9. All
samples should be disposed of in a manner that will inactivate
viruses.
*0. Solid
Waste: Autoclave *0 min. at **1°C.
*1. Liquid
Waste: Add sodium hypochlorite to a final concentration of 1.0%.
The waste should be allowed to stand for a minimum of *0 minutes
to inactivate the viruses before disposal.
*2. Substrate
Solution is easily contaminated. If bluish prior to
use,do not
use
| Country: |
China |
| Model No: |
E01A0002
|
| FOB Price: |
(Negotiable)
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| Place of Origin: |
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| Minimum Order Quantity: |
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| Product Group : |
Shanghai Bluegene Biotech
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